Early Poly Mass Effect (trimethylene-sebasat) On Biodegradation Rate In Liquid Media Using Mucor Miehei In Aerobic

Akhmad Kholisul Fuad, Diah Mardiana, Anna Roosdiana

Abstract


The author has conducted research about the effect of poly(trimethylene-sebacate) mass toward the rate of biodegradation using Mucor miehei in aerobic liquid media. Poly(trimethylene-sebacate) is a biodegradable linear aliphatic polyester, that can be degraded by Mucor miehei lipase. To determine the effect of poly(trimethylene-sebacate) mass in the biodegradation, the mass of poly(trimethylene-sebacate) were varied 0.06 g, 0.08 g, 0.1 g, 0.12 g and 0.14 g. Biodegradation process carried out for 12 hours, using liquid of Complex media, which was nutrient rich for Mucor miehei growth, and solution at pH 5. The resulting CO2 gas was flowed into the 50 mL reservoir of 0.1 M NaOH, followed by titration using 0.05 M HCl and MO (methyl orange) and PP (phenolptalein) indicator. The rate of CO2 gas were 0.287 x 10-3 M/h; 0.102 x 10-3 M/h; 0.137 x 10-3 M/h; 0.016 x 10-3 M/h; and 0.039 x 10-3 M/h respectively. The greater rate of CO2 produced the lower mass of poly(trimethylene-sebacate).


Keywords


aerobic, Poly(trimethylene-sebacate), biodegradation rate , Mucor miehei

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References


Ahmann, D dan Dorgan J. R. (2009), Bioengineering for Pollution Prevention through Development of Biobased Energy and Materials State of the Science Report, EPA/600/R-07/028, p.76-78.

Evita, Ci, Efrida M, Rofiq So, Lies Di, Hardaningpranamudai & Yutaka T (2000), Kerentanan Poliester Alifatik Terhadap Biodegradasi Biodegradability ofAliphatic Polyester, Jurnal Mikrobiologi Indonesia, 32-35.

Steven, M.P. (2001), Kimia Polimer, Cetakan pertama. Diterjemahkan oleh Sopyan L. PT Pradnya Paramita, Jakarta.

Belal, E.A. (2003), Investigations on The Biodegradation of Polyesters by Isolated Mesophilic Microbes. Dissertation, Technischen Universität Carolo- Wilhelmina.

Jay, J. M. (1991), Modern Food Biotechnology, 4th ed. Van Nostrand Reinhold, New York.

Ciptawati, E. (2009), Biodegradasi Poli(Trimetilen-Sebasat) Dalam Media Cair Yang Mengandung Mucor miehei, Skripsi, FMIPA Universitas Brawijaya, Malang.

Anggraini, S.D. (2010), Laju Pembentukan Gas CO2 Pada Biodegradasi Poli(trimetilen-sebasat) Secara Aerob Dalam Media Cair Yang Mengandung Mucor Miehei. Skripsi, FMIPA Universitas Brawijaya, Malang.

Permatasari, E.F. (2002), Isolasi dan Karakterisasi Ekstrak Kasar Enzim Lipase dari Mucor miehei. Skripsi, FMIPA Universitas Brawijaya, Malang.

Waluyo, L. (2004), Mikrobiologi Umum, UMM Press, USA

Monod, J. (1949) The growth of bacterial cultures, Ann. Rev. Microbiol., 3, 371-394.

Manfaati, R. (2010), Kinetika Dan Variabel Optimum Fermentasi Asam Laktat Dengan Media Campuran Tepung Tapioka Dan Limbah Cair Tahu Oleh Rhizopus Oryzae, Tesis, Universitas Diponegoro Semarang.

Muller, R.J. (2006), Biological Degradation of Synthetic Polyester-Enzimes as Potential Catalyst for Polyester Recycling, Process Biochemistry Mei 2006, 41(6): 2124-2128.

Bakar, A.R.D., I. Ahmad, and A. Ramli (2006), Chemical Recycling of Pet Waste from Soft Drink Bottles to Produce a Thermosetting Poliester Resin, Malaysia J. Chem., 8(1): 022-026.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2012.001.03.10

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