Analysis of Potential Differential Characteristics of Albumin Membranes and Vitelin Membranes Quail Eggs (Cortunix cortunix) Due to Hydrogen Peroxide Fluid

Ummu Hidayati Mardiyah, Didik Rahadi Santoso, Unggul Pundjung Juswono

Abstract



The research of analysis the potential difference characteristic of albumin and vitelline membranes quail egg (Coturnix Coturnix) due to contaminated by hydrogen peroxide (H 2 O 2 ) was conducted to determine the effect of hydrogen peroxide (toxic) against the resistance of a cell. Hydrogen peroxide (H 2 O 2 ) is one group of reactive oxygen species (ROS) that can damage lipids, proteins and nucleic acids because they can diffuseion freely passed by bilayer membrane, so that the hydrogen peroxide concentration needs to be controlled. The resistance of a cell can be determined from changes in the value of the measured membrane potential. The method used in the research applied a microelectrode connected with unity gain differential amplifier and PicoScope. The measurement results show that there is a change the potential difference at the albumin membrane and vitellin membrane of quail egg due to contaminated hydrogen peroxide (H 2 O 2 ). The highest value of the membrane potential difference in the concentration 0 ppm at the albumin membrane is (-144 ± 2 mV) and the vitelline membrane is (-172 ± 3 mV), while the lowest value of the potential difference in the concentration 4000 ppm is (-8 ± 2 mV) at the albumin membrane and (-18 ± 1 mV) at the vitellin membrane.


Keywords


potential difference, albumin membrane, vitelline membrane, quail egg, hydrogen peroxide solution (H202)

Full Text:

PDF

References


Hille, B. (2001) Ion Channels of Excitable Membranes. 3rd ed. Sinauer Associates, Sunderland, Massachusetts USA.

Pareja, D.A.G. (2011) Pharmacological Evaluation of New Meta- and ParaHydroxyl Substituted C-4-aryl-1 , 4dihydropyridines in Cardiomyocytes. Universidad De Chile.

Blatt, M.R., Beilby, M.J. and Tester, M. (1990) Voltage dependence of the Chara proton pump revealed by current-voltage measurement during rapid metabolic blockade with cyanide. The Journal of Membrane Biology, 114, 205–23.

Cuzzocrea, S., Riley, D.P., Caputi, A.P. and Salvemini, D. (2001) Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacological Reviews, 53, 135–59.

Matondang, R.A., Rochima, E. and Kurniawati, N. (2015) Studi Kandungan Formalin Dan Zat Pemutih Pada Ikan Asin Di Beberapa Pasar Kota Bandung. Perikanan Kelautan, 6, 70–7.

Miller, A.J., Cookson, S.J., Smith, S.J. and Wells, D.M. (2001) The use of microelectrodes to investigate compartmentation and the transport of metabolized inorganic ions in plants. Journal of Experimental Botany, 52, 541–9.

Bienert, G.P., Schjoerring, J.K. and Jahn, T.P. (2006) Membrane transport of hydrogen peroxide. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1758, 994–1003. https://doi.org/10.1016/j.bbamem.2006.02.0 15

Latifah, R. (2011) Pengaruh Merkuri Klorida, Timbal Nitrat dan Hidrogen Peroksida Terhadap Potensial Sel Telur Ikan Gurami Studi Kasus Polutan Air di Daerah Aliran Sungai (DAS) Brantas. Universitas Brawijaya.

Ningsih, I.S., Juswono, U.P. and Kusharto. (2013) Pengaruh Pemberian Ekstrak Teh Hijau Terhadap Potensial Membran Sel Telur Ikan Nila (Oreochromis niloticus) Yang Tercemari Kelompok Senyawa Oksigen Reaktif (ROS) Berupa Hidrogen Peroksida (H2O2). Physics Student Journal, 1, 32–5.

Kappen, B. (2008) Introduction to Biophysics. University Nijmegen, Netherlands.

Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter., P. (2002) Molecullar Biology of The Cell. 4th ed. Garland Science, New York.

Ikawati, Z. (2008) Pengantar Farmakologi Molekuler. Gadjah Mada University Press, Yogyakarta.

Suryohudoyo, P. (2000) Oksidan, Antioksidan dan Radikal Bebas. Buku Naskah Lengkap Simposium Pengaruh Radikal Bebas Terhadap Penuaan (Lustrum IX FKUA 1995-2000) 7 September 2000, Universitas Airlangga, Surabaya.

Paulsen, C.E. and Carroll, K.S. (2013) Cysteine-Mediated Redox Signaling: Chemistry, Biology, and Tools for Discovery. Chemical Reviews, 113, 4633– 79. https://doi.org/10.1021/cr300163e

Handoko, E. and Sumilat, W.A. (2011) Metabolisme Hidrogen Peroksida dan Peranannya pada Infeksi Telinga [Internet]. PERHATI-KL.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2017.004.01.10

Refbacks

  • There are currently no refbacks.