Antagonist Mold Exploration of Phytophthora spp. Pathogen on Apple Plant

Meisarina Nugrahani, Suharjono Suharjono, Otto Endarto

Abstract


The attack of Phytophthora spp. caused the descrease of apple crops production up to 90 %. Some antagonist mold species are able to inhibit the growth of Phytophthora pathogens onapple crops. The objectives of this research were to identifify and to study the potency of antagonist mold for inhibite the growth of Phytophthora spp. pathogens on apple crops. The Phytophthora spp. were isolated using baiting method with apple fruit  while antagonist molds were isolated by dilution method from soil sample of apple plantation. The inhibition assay of antagonist molds against Phytophthora was carried out by dual culture method. The isolated both of Phytophthora spp. and antagonist mold isolates were identified based on phenotype characters. The research result showed six molds namely Trichoderma sp.1, Trichoderma sp.4, Trichoderma sp.6, Aspergillus sp.2, Aspergillus sp.3 and Penicillium sp.1 which were antagonist on the Phytophthora pathogens onapple crops. Among the antagonist mold species, Trichoderma sp.6 had the highest inhibitory potency (62.95 %) while Penicillium sp.1 had the lowest inhibitory potency (28.82 %) against the growth of Phytophthora


Keywords


antagonist, apple, mold, Phytophthora sp

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References


Triwiratno, A. (2008), Koleksi Varietas BaruApel Dari Negara Belanda, Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro), Batu.

Drenth and D. I. Guest (2004), Diversity and Management of Phytophthora in Southeast Asia,ACIAR Monograph, No. 114.

Hartman, J., J. Beale and P. Bachi(2008), Root and Collar Rots of Tree Fruits, Plant Pathol Fact Sheet. University of Kentucky, UK.

Miyake, Y., J. Sakai, M. Shibata, N. Yonekura, I. Miura, K. Kumakura and K. Nagayama(2005), Fungicidial Activity ofBenthiavalicarb-isopropyl against Phytophthora infestans and Its Controlling Activity Against Late Blight Disease,J. Pestic. Sci., 30 (4): 390-396.

Aryantha, I. N .P. and D. I. Guest(2006), Mycoparasitic and Antagonistic Inhibition on Phytophthora cinnamomi,Plant Pathol. J., 5 (3): 291-298.

Gisi, U. and Y. Cohen(1996), Resistance to Phenylamide Fungicides: A Case Study with Phytophthora infestans Involving Mating Type and Race Structure,Ann. Rev. Phytopathol,34: 549-72.

Muryati, L. Octriana, D. Emilda, P.J. Santoso and D. Sunarwati (2009), Effect Of Organic Fertilizers On Susceptibility Of Potted Durian Seedlings To Phytophthora Diseases,J. Fruit and Ornamental Plant Research,17(1): 67-77.

Kwon, J. H., H. J. Jee, S. S. Shen and Y. S.Chae (2007), Phytophthora Rot of Broad Bean (Vicia faba) Caused by Phytophthora nicotianae in Korea,Plant Pathol. J.,23(1) : 31-33.

Funder, S. (1953), Practical Mycology: Manual For Identification of Fungi, Hafner Publishing Company, New York.

Koneman, E. R., G. D. Roberts and S. A. Wright (1978),Practical Laboratory Mycology, Preston Street, USA.

Harris, J. L. (1986), Modified Method for Fungal Slide Culture,Clinical Microbiol. 24 (3): 460-461.

Suharjono (2008), Keanegaraman dan Potensi Pseudomonas Strain Indigenous Pendegradasi Surfaktan Anionik di Ekosistem Sungai Tercemar Detergen, Disertasi, Fakultas Biologi, Universitas Gajah Mada, Yogyakarta.

Mpika,J., I. B. Kébé1, A. E. Issali, F. K. N’Guessan, S. Druzhinina, M. Komon-Zélazowska, C. P. Kubicek and S. Aké. (2009),Antagonist Potential of Trichoderma Indigenous Isolates for Biological Control of Phytophthora Palmivora The Causative Agent of Black Pod Disease on Cocoa (Theobroma Cacao L.) in Côte d’Ivoire,African J. Biotechnol., 8 (20): 5280-5293.

Suharjono, T. H. Kurniati, Soejono and S. Dewi (2004), Uji Antagonis Trichoderma sp. dan Gliocladium sp. terhadap Fusarium Penyebab Penyakit Layu pada Beberapa Jenis Tanaman Pisang di Kebun Raya Purwodadi secara in-vitro, Biota IX, (2): 119-124.

Singh, A. and M.N. Islam (2010),In Vitro Evaluation of Trichodermaspp. Against Phytophthora nicotianae, Int. J. Expt. Agric., 1(1): 20-25.

Yuleli (2009), Penggunaan Beberapa Jenis Fungi Untuk Meningkatkan Pertumbuhan Tanaman Karet (Hevea Brasiliensis) di Tanah Gambut, Tesis, Program Studi Biologi, Sekolah Pascasarjana Universitas Sumatera Utara, Medan.

Noveriza, R. and T. H. Quimio (2004), Soil Mycroflora of Black Pepper Rhizospere In The Philippine And Their In Vitro Antagonism Againt Phytophthora capsici L. Indonesian J., Agric. Sci., 5(1) 2004:1-10.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2012.001.03.4

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