Calcium Oxalate Crystals (CaOx) on Porang (Amorphopallus muelleri Blume) Exposed and Unexposed Sun

Nurul Chairiyah, Nunung Harijati, Retno Mastuti

Abstract


Sunlight was suspected to affect CaOx crystal formation in porang. This research had aim to determine variations in forms of CaOx crystals and the influence of shade on the density of CaOx crystals in porang. Preparations for microscopic observation derived from sliced leaf, petiole, and tuber of porang that grown under shaded and exposed to sunlight conditions. Sliced organ was cleared by using the modified clearing method. The parameters that observed included shape, shape variety, and density of CaOx crystals. CaOx crystal density were analyzed using ANOVA followed by Tukey (α 0.05). The differences of crystal density between the edges and middle of the organ were analyzed using Paired Samples T Test. Microscopic observation showed that CaOx crystals were grouped into large (20-710 m) and small (1-15 m) crystals size. The density of CaOx crystals in plants exposed to sunlight was 3 times higher than the shaded plants. Leaf organ had the highest number of crystal compared to others organ. The tuber had the lowest density of CaOx crystals among organ. In addition, the shaded or exposed to sunlight condition had no effect on CaOx crystal density between the edges and center of the organ.


Keywords


Porang, Shade, CaOx crystals, Density of crystals, Variation shape of crystals

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References


Pitojo, S. (2007), Suweg, Kanisius, Yogyakarta, pg. 37- 44.

Ilarslan, H., R. G. Palmer, J. Imsande, and H. T. Horner (1997), Quantitative Determination of Calcium Oxalate and Oxalate in Developing Seeds of Soybean (Leguminosae), American Journal of Botany, 84(9): 1042–1046.

Franceschi, V.R., and P.A. Nakata (2005), Calcium Oxalate in Plant: Formation and Function, Annual Review of Plant Biology; 56: 41-71.

Prychid, C.J. and P.J. Rudall (1999), Calcium Oxalate Crystals in Monocotyledons: A Review of Their Structure and Systematics, Annals of Botany, 84: 725 – 739.

Cao, H. (2003), The Distribution of Calcium Oxalate Crystals in Genus Dieffenbachia Schott and The Relationship Between Environmental Factors and Crystal Quantity and Quality, University of Florida, pg. 22.

Seal, S.N. and S.P. Sen (1970), The Photosynthetic Production of Oxalic Acid in Oxalis corniculata, Plant and Cell Physiology, 11: 119-128.

Moreau, A.G. and G.P. Savage (2009), Oxalate Content of Purslane Leaves and The Effect of Combining Them with Yoghurt or Coconut Products, Journal of Food Composition and Analysis, 22: 303–306.

Libert, Bo and V.R. Franceschi. 1987. Oxalate in Crop Plants. Journal of Agricultural and Food Chemistry, 35: 926-938.

Webb, M. C. 1999. Cell-Mediated Crystallization of Calcium Oxalate in Plants. The Plant Cell, 11: 751–761.

Ji, X. and X. Peng. 2005. Oxalate Accumulation as Regulated by Nitrogen Forms and Its Relationship to Photosynthesis in Rice. Journal of Integrative Plant Biology (Formerly Acta Botanica Sinica). 47 (7): 831-838.

Korth, K.L., S.J. Doege, S.H. Park, F.L.Goggin, Qin Wang, S.K.Gomez, G. Liu, L.Jia, and P.A.Nakata. 2006. Medicago truncatula Mutants Demonstrate The Role of Plant Calcium Oxalate Crystals as An Effective Defense Against Chewing Insects1. Plant Physiology, 141: 188–195.

Ilarslan, H., R.G. Palmer, and H.T. Horner. 2001. Calcium Oxalate Crystals in Developing Seeds of Soybean. Annals of Botany. 88:243-257.

Kuo, L., Maurice, and V.R. Franceschi.2007. Correlations Between Calcium Oxalate Crystals and Photosynthetic Activities in Palisade Cells of Shade Adapted Peperomia glabella. Botanical Studies, 48: 155-164.

Kisaki, T., and N.E. Tolbert. 1969. Glycolate and Glyoxylate Metabolism by Isolated Peroxisomes or Chloroplasts. Plant Physiology. 44: 242-250.

Khan, M.S. 2007. Engineering Photorespiration in Chloroplasts: a Novel Strategy for Increasing Biomass Production. TRENDS in Biotechnology. 25 (10): 437-440.

Lindqvist, Y., and C. Brändén. 1985. Structure of Glycolate Oxidase from Spinach. Proceedings of The National Academy of Sciences. USA. 82: 6855-6859.

Sakai, W.S., M. Hanson, and R.C. Jones. 1972. Raphides with Barbs and Grooves in Xanthosoma sagittifolium (Araeae). Science. 178: 314 -315.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2011.001.02.6

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