Drought Resistance Variation of Mutant of Kenaf KR11 Based on Prolin Accumulation
Abstract
Kenaf is a fiber plants of the family Malvaceae. KR 11 is a high yielding kenaf variety which is resistant to drought stress. Mutations by EMS at several species known to increase resistance to drought stress by accumulation of osmolite. In this research, physiological testing on 5 mutants to determine the effect of the mutation on the drought stress response of kenaf KR 11was conducted. Experiment were done by comparing the physiological accumulation of proline in normal and drought stress conditions. The plants were watered every 3 days for 14 days with 100% of field capacity as normal conditions and 25% field capacity as drought stress. The results showeddifferences inthe pattern ofprolineaccumulationbetweencontrolandmutant in normal and drought stress condition. While control KR11 increased the accumulation on drought condition, mutants showed decreasedprolin content under conditions of stresswith varying decreasing levels. The mostsignificant pattern ofproline accumulationcompared with control, is shownby themutant4and5. Prolin accumulation pattern is controlled by the proline metabolism control genes, P5CS and ProDH and its feedback regulation. Alteration of this pattern is most likely an effect of mutation induction on the mutant sample.
Keywords
Full Text:
PDFReferences
Irawati, Fenny (2002), Pengaruh vitamin C dan E terhadap kuantitas eritrosit, FMIPA Universitas Brawijaya, Malang.
Prabhakar, K.R., V.P. Veerapur, et al. (2006), Evaluation and Optimization of Coronopusdidymus Linn in gamma irradiated mice, Radioprotective Activity of International Journal of Radiation Biology.
Ruslan, B.I. Wahyudi, et a, Info Nuklir, Pusat Diseminasi Iptek Nuklir BATAN, Jakarta.
Levine, M., K.R. Dhariwal, R.W. Welch, Y. Wang, dan J.B. Park (1995), Determination of Optimal Vitamin C Requirements in Human dalam: The WA MERICAN Journal of Clinical Nutrition, 62(Suppl) 1347S-1356S.
Sies, H (1991), Oxidative Stress: From Basic Research to Clinical.
Hariyatmi (2004), Kemampuan Vitamin E Sebagai Antioksidan Terhadap Radikal Bebas Pada Lanjut Usia, Surakarta, Jurnal MIPA , vol 14 No.1.
Pramono (2012), Nutrisi Massa Menopause Mencegah Osteoporosis, DOI: www.rsulin.com. accessed at July 10th 2012.
Astuti, S (2008), Isoflavon Kedelai dan Potensinya Sebagai Penangkap Radikal Bebas. Teknologi Industri dan Hasil Pertanian 13.
Murray, R. K., Granner, D.K., Mayes, P.A., Rodwell, V.W (2003), Harper’s Biochemistry, 6th Edition, Lange Medical. California.
Miller (1998), Effect of Dietary Patterns on Measures of Lipid Peroxidation: Results From A Randomized Clinical Trial, Circulation, 98: 2390-2395.
BATAN (2000), Materi Diklat Petugas Proteksi Radiasi Bidang Radiodiagnostik. Jakarta.j
Alatas, Z (2006), Efek Pewarisan Akibat Radiasi Pengion, Buletin ALARA. 8(2).
Allen, R.G., Tressini, M (2000), Oxidative Stress and Gene Regulation. Free Radical Biol Med, 28, 463-499.
Maulida, F. (2010), Efek ekstrak daun krokot (portulaca oleracea l.) Terhadap Kadar Alanin Transaminase (alt) Tikus Putih (rattus norvegicus) yang Diberi Minyak Goreng Deep Fying Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta.
Kreshnamurti, I (2012), Radioterapi Pada Kanker Serviks, Departemen Obstetri dan Ginekologi Fakultas Kedokteran Universitas Sriwijaya Palembang.
Rejon, R.F., Pena, G.M., Geanado ,F., Galiana, R.J., Blanco, I., Olmeidilla, B, (2003), Plasma status of retinol, alpha and gamma tochopherols and main carotenoids to first myocardial infarction: case control and follow up study, Nutrition., 18, 26-31.
Davies, M.B., Austin, J., dan Partridge, D.A., Vitamin C: its chemistry and biochemistry. Royal Society of Chemistry.
Suhartono E, Fachir H & Setiawan B. (2007), Kapita Sketsa Biokimia Stres Oksidatif Dasar dan Penyakit, Universitas Lambung Mangkurat, Banjarmasin: Pustaka Benua.
Winarsi, Hery (2007), Antioksidan Alami dan Radikal Bebas Potensi dan Aplikasi dalam Kesehatan, Kanisius, Yogyakarta.
Winarsi, H., D. Muchtadi, F.R. Zakaria and B. Purwantoro (2004), Respon Hormonal-Imunitas Wanita Premenopause yang Diintervensi Minuman Fungsional Berbasis Susu Skim Yang Disuplementasi dengan 100 mg Isoflavon Kedelai Dan 8 mg Zn-sulfat (SUSUMENO), Teknol. dan Industri Pangan. XV: 28-34.
DOI: http://dx.doi.org/10.21776/ub.natural-b.2014.002.03.10
Refbacks
- There are currently no refbacks.