Study of Stearic Acid Layer (SA) Microstructure on Surface Quartz Crystal Microbalance (QCM) Sensors

Nurramdaniyah Nurramdaniyah, Masdiana Padega, Djoko Herry Santjojo, Setyawan P. Sakti, Masruroh Masruroh

Abstract


In the development of QCM-based biosensor sensors, the sensor surface is a very important part because it determine how the surface interacting with its environment especially during the immobilization of biomolecules process. In the utilization of QCM as biomolecule detecting biosensor, it is necessary to coat the polymer material on the surface of QCM. A stearic acid layer is used as an immobilization matrix layer because stearic acid has both polar and non polar properties so it can interact with biomolecules. The stearic acid layer is deposited on the polystyrene surface using a vacuum evaporation method. A study of the microstructure of the stearic acid coating was conducted to determine the possibility of the deposited stearic acid layer used as the matrix layer. In this research, microstructural study of layers of the stearic acid by using Scanning Electron Microscope (SEM) was carried out to find out the surface morphology of stearic acid. X-Ray Difraction (XRD) measurements were conducted to identify the crystal structure of stearic acid while Fourier Transform Infrared (FTIR) measurements were used to determine the stearic acid functional group. The Scanning Electron Microscope (SEM) results show the surface of the cavity and pore layers of the polystyrene layer was coated with white fibers homogeneously deposited on the surface of QCM / Polystyrene. Furthermore, the X-Ray Difraction (XRD) results showing a spectrum peak at an angle of 2q = 26,66° indicates the existence of stearic acid crystals. Whereas, the measurement results of the Fourier Transform Infrared (FTIR) indicate the appearance of C-H functional groups at a wavelength value of 3600-2600 cm-1 which indicates the functionality of the stearic acid layer.

 


Keywords


Stearic acid layer, Morphology, Crystal structure, C-H

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References


H. Jie, “Technical background , applications and implementation of quartz crystal microbalance systems,” no. September, 2006.

A. Katerkamp, R. Renneberg, and K. Cammann, “Surface investigations on the development of a direct optical,” vol. 13, pp. 1141–1147, 1998.

D. J. D. H. Djoko, L. A. Didik, E. Rahmawati, M. Pagaga, and S. P. Sakti, “Solvent effect on morphology of polystyrene coating and their role to improvement for biomolecule Immobilization in application of QCM based biosensor,” vol. 531, pp. 54–57, 2014.

Y.-C. Lin, Y.-C. Chen, and L.-Y. Chen, “Effect of Electrode Surface Modification by Sulfide on QCM Based Protein Biosensor,” Opt. Photonics J., vol. 03, no. 02, pp. 305–307, 2013.

B. B. Prasad and I. Pandey, “Molecularly imprinted polymer-based piezoelectric sensor for enantio-selective analysis of malic acid isomers,” Sensors Actuators, B Chem., vol. 181, pp. 596–604, 2013.

S. M. Reddy, Q. T. Phan, H. El-Sharif, L. Govada, D. Stevenson, and N. E. Chayen, “Protein Crystallization and Biosensor Applications of Hydrogel-Based Molecularly Imprinted Polymers,” Biomacromolecules, vol. 13, no. 12, pp. 3959–3965, 2012.

H. F. El-Sharif, H. Aizawa, and S. M. Reddy, “Spectroscopic and quartz crystal microbalance (QCM) characterisation of protein-based MIPs,” Sensors Actuators, B Chem., vol. 206, pp. 239–245, 2015.

S. P. Sakti, D. J. D. H. Santjojo, and S. N. Saputri, “Improvement of Biomolecule Immobilization on Polystyrene Surface by Increasing Surface Roughness,” vol. 3, no. 3, pp. 3–7, 2012.

Masruroh, D. J. D. H. Djoko, S. Rahayu, and S. P. Sakti, “Viscoelastic and Morphological Behavior of Stearic Acid Layer on Top of Polystyrene as Immobilisation Matrix for QCM Sensor,” Mater. Sci. Forum, vol. 848, pp. 757–762, 2016.

K. Peng, J. Zhang, H. Yang, and J. Ouyang, “RSC Advances Acid-hybridized expanded perlite as a composite phase-change material in wallboards,” RSC Adv., vol. 5, pp. 66134–66140, 2015.

B. H. Stuart, Infrared Spectroscopy: Fundamentals and Applications, vol. 8. 2004.

L. Robinet and M. Corbeil, “The Characterization of Metal Soaps,” Stud. Conserv., vol. 48, no. 1, pp. 23–40, 2003.

R. S. Andriamitantsoa, W. Dong, H. Gao, and G. Wang, “Porous organic–inorganic hybrid xerogels for stearic acid shape-stabilized phase change materials,” New J. Chem., vol. 41, no. 4, pp. 1790–1797, 2017.

S. J. Lee and K. Kim, “Diffuse reflectance infrared spectra of stearic acid self-assembled on fine silver particles,” Vib. Spectrosc., vol. 18, no. 2, pp. 187–201, 1998.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2017.004.02.4

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